Nikon is one of the most recognizable names in cameras but it is also one of the leading manufacturers of biological microscopy, offering high quality optical systems and cutting edge features. From compact models to the more sophisticated units, Nikon’s biological microscopes are one of the most reliable and ergonomically designed. Here are some guide questions and answers on how to use and care for your own unit:

Securing the specimen in place
It’s always a good idea to secure the specimen when working. Even small movements can nudge the slide out of focus, which may become a problem when you’re using high magnifications. Nikon biological microscopes use a springloaded holder to secure the slide in place.

Magnification to use when viewing a specimen
Always use the objective with the lowest power first. This will help you find the specimen under the microscope more easily because this objective has a wide field of view. Once you have located your specimen, simply adjust the magnification or rotate the objective until you find the image or magnification that you like best. When rotating the nosepiece, make sure it clicks into place so it is focused securely on the specimen.

Adjusting the microscope according to user’s build
Nikon biological microscopes may be adjusted according to the height of the user by simply manipulating the length of the eyepiece. This helps eliminate strain and discomfort during use. The eyepiece tube is tilted naturally at a 30 degree angle for comfortable viewing, although it may be adjusted to conform to a specific user. This should offer the best angle while encouraging a natural posture for the body. If the user is very tall, an eye level riser may be used to raise the eye point. Eye level risers are 25mm high and Nikon’s biological microscopes allow the use of up to 2 risers.

Using light
A Nikon biological microscope is equipped with a built-in halogen lamp to facilitate viewing. If there is no source of power, use the mirror unit and simply find a source of light. The mirror is detachable. To install, remove the field lens unit and attach to secure the mirror in place. The mirror unit will give a decent amount of illumination for viewing but don’t expect too much during high magnifications where it may not perform as well.

Using the focus knobs
If you want to view objects using low to medium magnifications, use the coarse focus knob. If you want a closer look using high magnifications, use the fine focus knob. At very high magnifications, it’s usually more difficult to focus the objective lens that is why you should try viewing the specimen with the coarse focus first before refining the image with the fine focus knob.

Working with short working distance objectives
A problem with short working distance objectives is that there is always the risk they will hit the slide and damage it. With a Nikon biological microscope, you can prevent this from happening by setting the upper limit to the stage movement first. Even if you changed slides, you can keep the specimen and the objective safe.

Attaching a phase contrast accessory
If you want to enhance image clarity, insert the slider (usually a single phase annulus) into the condenser. This automatically opens the aperture diaphragm. Simply choose which ring stop or phase contrast slider you want to use for which objective.

Using the microscope with a digital camera
A digital camera may be attached if you use the trinocular eyepiece tube. You can either view the images using a computer or a camera with a built-in LCD monitor. A digital camera can facilitate better viewing for presentations and also allow storage of images.

Using accessories
Nikon biological microscopes often have accompanying accessories for a particular series or model. If you’re buying an accessory from a different series or if you’re using a different brand, check for compatibility first.

Caring for the microscope
Your microscope may probably be made with tough and reliable materials but it is still a very sensitive instrument. Regular cleaning and common sense care and maintenance should keep it in top shape. Never touch the objective lens. If you don’t scratch them, your fingers will leave dirt and oil on the lens, which will be particularly difficult to clean. Regularly wipe using a lens cloth or tissue and don’t use a damp towel or cloth. When not in use, store the microscope in a cool, dark place away from direct sunlight and moisture.

Handling molds
Nikon’s biological microscopes are coated with anti-mold paint that helps prevent mold presence even in environments with high humidity. Some microscope units can even last for about 3 years at medium to high exposures during tests. To ensure that your unit is safe, follow storage recommendations.

Transporting the microscope
When traveling or moving the microscope to another area, it’s always a good idea to lock the eyepiece in place. This helps prevent loss, theft or damage. For long distances, keep the microscope in a storage case during transit.

Troubleshooting
Always read the manuals before using the equipment. This will ensure that you will use the unit correctly and will still be covered by the warranty. When troubleshooting, refer to the manual. Never try to repair a Nikon biological microscope by yourself. You will void the warranty and cause more damage.